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Label-free 3D Live Cell Imaging and Quantification using Holotomography
Manage episode 411329979 series 3391756
Bitesize Bio에서 제공하는 콘텐츠입니다. 에피소드, 그래픽, 팟캐스트 설명을 포함한 모든 팟캐스트 콘텐츠는 Bitesize Bio 또는 해당 팟캐스트 플랫폼 파트너가 직접 업로드하고 제공합니다. 누군가가 귀하의 허락 없이 귀하의 저작물을 사용하고 있다고 생각되는 경우 여기에 설명된 절차를 따르실 수 있습니다 https://ko.player.fm/legal.
Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation.
It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets.
Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.
This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens.
Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.
Watch the full presentation here: https://events.bitesizebio.com/label-free-3d-live-cell-imaging-and/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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